Coding
Part:BBa_K5034209:Design
Designed by: Zongyu Guo Group: iGEM24_Nanjing-China (2024-09-16)
Poly P -> Poly P(smaller) or Pi
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in S. oneidensis and regulating cellular phosphorus metabolism and electron transfer.
Source
Endopolyphosphatase(PPN1) from Saccharomyces cerevisiae. NCBI reference sequence: NC_001136.10.
References
Lichko, L. P., Kulakovskaya, T. V., & Kulaev, I. S. (2006). Inorganic polyphosphate and exopolyphosphatase in the nuclei of Saccharomyces cerevisiae: dependence on the growth phase and inactivation of the PPX1 and PPN1 genes. Yeast, 23(10), 735-740. doi:10.1002/yea.1391